In collaboration with Klaus Dreisewerd (Institute for Medical Physics and Biophysics) a novel ion source was developed for atmospheric pressure mass spectrometry (S. König, A. Pirkl,
K. Dreisewerd).
Since 1999 there is a successful collaboration with
Xantec bioanalytics Münster using alternative surfaces for surface
plasmon resonance and microaffinity capture mass spectrometry (MAC-MS). A special flow cell was developed to interface both techniques.
The team was extended by Rafal Marzalek, (Erasmus, University of Krakow), Oliver Kollas and Klaus Dreisewerd
(Medical Physics and Biophysics) in 2006. This allowed comparative investigations of axial
and orthogonal TOFs. In addition, IR-MALDI is tested with an ion trap.
Reference: König, S., Kollas, O., Dreisewerd, K. Generation of highly-charged peptide and protein ions by atmospheric pressure matrix-assisted infrared laser desorption
ionization ion trap mass spectrometry.
Anal. Chem. (2007) 79, 14: 5484 -5488
Institut für Pharmazeutische und Medizinische Chemie
König, S., Hasche, A., Pallast, S., Krieglstein, J., Klumpp, S. Detection of ATP-binding to growth factors. JASMS (2008) DOI: 10.1016/j.jasms.2007.10.010
Tessmer, N., König, S., Malkus, U., Reichelt, R., Pötter, M., Steinbüchel, A. Heat-shock protein HspA mimics the function of phasins sensu strictu in recombinant strains of Escherichia
coli accumulating polythioesters or polyhydroxyalkanoates.
Microbiology (2007) 153,2:366-374
Zeller, M., M. Pötter, A. Steinbüchel, F. Reinecke, W. I. Burkitt, P. Derrick, and S. König »Mass spectrometry identifies homologues of phasin PhaP1 protein of Ralstonia eutropha on
polyhydroxybutyrate granules«, Biomacromolecular Mass Spectrometry 1, 1 (2005) 75-84
König, S., Mehlich, A.-M., Büllesbach, J., Michiels, N. »Allohormones in Lumbricus terrestris? Mass spectrometry of the setal gland product indicates possible role of ubiquitin« Invertebrate
Reproduction & Dev. 49, 1-2 (2006) 103-111
SwissProt submission P84589
Bettina Zeiss, Ralph Pirow
DGMS 2003
König, S., M. Zeller, J. Grote, B. Zeis, and R. Paul »Identification and Sequencing of Daphnia magna Hemoglobins Using MALDI Peptide Mapping and cap– and nanoHPLC-MS/MS«
de novo Sequenzierung bei Champignon-Tyrosinase und Aureusidinsynthase